Enterococcus na-eguzogide Vancomycin na Gene na-eguzogide ọgwụ

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke vancomycin na-eguzogide enterococcus (VRE) na mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ VanA na VanB na sputum mmadụ, ọbara, mmamịrị ma ọ bụ obodo dị ọcha.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-OT090-Vancomycin na-eguzogide Enterococcus na ngwa nchọpụta Gene na-eguzogide ọgwụ (Fluorescence PCR)

Epidemiology

A na-akpọkwa iguzogide ọgwụ ọjọọ dị ka nguzogide ọgwụ, na-ezo aka nguzogide nje bacteria na omume nke ọgwụ nje.Ozugbo nguzogide ọgwụ na-apụta, mmetụta chemotherapy nke ọgwụ ga-ebelata nke ukwuu.A na-ekewa nguzogide ọgwụ na nkwụsi ike dị n'ime yana nguzogide enwetara.A na-ekpebi nguzogide dị n'ime ya site na mkpụrụ ndụ ihe nketa chromosomal nke nje, na-ebufe site n'ọgbọ ruo n'ọgbọ, ọ gaghị agbanwe.Nguzogide enwetara bụ n'ihi na mgbe emekọrịtachara ọgwụ nje, nje bacteria na-agbanwe ụzọ ndụ nke onwe ha ka ọgwụ nje ghara igbu ha.

Mkpụrụ ndụ ihe nketa vancomycin VanA na VanB na-enweta nguzogide ọgwụ, n'ime nke VanA na-egosi ọkwa dị elu nke iguzogide vancomycin na teicoplanin, VanB na-egosi ọkwa dị iche iche nke iguzogide vancomycin, ma na-enwe mmetụta nke teicoplanin.A na-ejikarị Vancomycin n'ụlọ ọgwụ na-agwọ ọrịa nje na-akpata Gram-positive, ma n'ihi mpụta nke vancomycin na-eguzogide enterococci (VRE), karịsịa enterococcus faecalis na enterococcus faecium, na-aza ihe karịrị 90%, ọ ewetala nnukwu ihe ịma aka ọhụrụ na ọgwụgwọ ụlọ ọgwụ. .Ka ọ dị ugbu a, ọ nweghị ọgwụ nje antibacterial akọwapụtara maka ọgwụgwọ VRE.Kedu ihe ọzọ, VRE nwekwara ike ibunye mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ na enterococci ndị ọzọ ma ọ bụ nje bacteria Gram-positive ọzọ.

Ọwa

FAM Enterococci na-eguzogide Vancomycin (VRE): Enterococcus faecalis na Enterococcus faecium
VIC/HEX Njikwa ime
CY5 vancomycin nguzogide gene VanB
ROX vancomycin mgbochi mkpụrụ ndụ ihe nketa VanA

Nka nka

Nchekwa Mmiri: ≤-18℃
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị sputum, ọbara, mmamịrị ma ọ bụ obodo dị ọcha
CV ≤5.0%
Ct ≤36
LoD 103CFU/ml
Nkọwapụta Ọ dịghị cross-reactivity na ndị ọzọ iku ume nje dị ka klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus. escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, iku ume adenovirus, ma ọ bụ sample nwere mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ CTX, mecA, SME, Nlereanya SHV na TEM.
Ngwa ndị ọdabara Ngwa Biosystem 7500 ezigbo oge PCR

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Usoro ọrụ

Ndị na-ahụ maka mmịpụta akwadoro: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .


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