Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke staphylococcus aureus na methicillin na-eguzogide staphylococcus aureus nucleic acids n'ụdị sputum mmadụ, imi imi na akpụkpọ anụ na anụ ahụ dị nro na-efe efe na vitro.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-OT062 Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus Nucleic Acid Detection Kit (Fluorescence PCR)

Asambodo

CE

Epidemiology

Staphylococcus aureus bụ otu n'ime nje bacteria na-akpata ọrịa nosocomial.Staphylococcus aureus (SA) bụ nke staphylococcus ma bụrụ onye nnọchianya nke nje bacteria Gram-positive, nke nwere ike ịmepụta nsị dị iche iche na enzymes na-emerụ ahụ.Nje bacteria nwere njirimara nke nkesa sara mbara, pathogenicity siri ike na oke nguzogide.Mkpụrụ ndụ ihe nketa nuclease (nuc) bụ mkpụrụ ndụ ihe nketa nke staphylococcus aureus echekwara nke ukwuu.

Ọwa

FAM gene mecA na-eguzogide methicillin
ROX

Njikwa ime

CY5 staphylococcus aureus nuc gene

Nka nka

Nchekwa ≤-18℃ & echebe site na ìhè
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị sputum, akpụkpọ ahụ na anụ ahụ dị nro ọrịa sample, na imi imi
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL nje bacteria na-eguzogide methicillin.Mgbe ngwa ahụ chọpụtara ntụnye aka LoD nke mba, enwere ike ịhụ 1000/mL staphylococcus aureus
Nkọwapụta Nnwale nlegharị anya nke cross-reactivity na-egosi na ngwa a enweghị ihe ọ bụla na-emeghachi omume na nje ndị ọzọ na-eku ume iku ume dị ka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, staphylococcus epidermidis na-eguzogide methicillin, pseudomonas aeruginosa, escherichia coli, kleetobisinia, pseudomonas aeruginosa. Ndị na-eme ihe na-eme ka ahụ dịkwuo mma, gụnyere streptococcus pneumonia, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, neisseria meningitidis, haemophilus influenzae.
Ngwa ndị ọdabara Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

QuantStudio®Sistemụ PCR 5 ezigbo oge

Sistemụ PCR SLAN-96P ezigbo oge

LightCycler®Sistemụ PCR 480 ezigbo oge

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Enwere ike iji Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-eji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Tinye 200µL nke saline nkịtị n'ime mmiri a na-ahazi, yana usoro ndị na-esote kwesịrị ịwepụta dịka ntuziaka ahụ si dị, na olu elution akwadoro bụ 80µL.

Nhọrọ 2.

Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 1mL nke saline nkịtị ka ọ na-asọpụta mgbe ịsachara ya na saline nkịtị, wee gwakọta nke ọma.Centrifuge na 13,000r/min maka nkeji 5, wepụ ihe dị elu (chekwaa 10-20µL nke supernatant), ma soro ntuziaka maka mmịpụta na-esote.

Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd.Ekwesịrị ịme mmịpụta ahụ nke ọma dịka nzọụkwụ 2 nke ntuziaka ntuziaka si dị.A na-atụ aro ka iji RNase na mmiri na-enweghị DNA maka elution na oke 100µL.

 


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