Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus Nucleic Acid.

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke staphylococcus aureus na methicillin na-eguzogide staphylococcus aureus nucleic acid na sputum sample mmadụ, akpụkpọ ahụ na anụ ahụ dị nro, na ọbara dum na vitro.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-OT062-Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus Nucleic Acid Detection Kit (Fluorescence PCR)

Asambodo

CE

Epidemiology

Staphylococcus aureus bụ otu n'ime nje bacteria na-akpata ọrịa nosocomial.Staphylococcus aureus (SA) bụ nke staphylococcus ma bụrụ onye nnọchianya nke nje bacteria Gram-positive, nke nwere ike ịmepụta nsị dị iche iche na enzymes na-emerụ ahụ.Nje bacteria nwere njirimara nke nkesa sara mbara, pathogenicity siri ike na oke nguzogide.Mkpụrụ ndụ ihe nketa nuclease (nuc) bụ mkpụrụ ndụ ihe nketa nke staphylococcus aureus echekwara nke ukwuu.N'afọ ndị na-adịbeghị anya, n'ihi iji hormones na ọgwụ mgbochi ọrịa na-eme ihe n'ụzọ na-ezighị ezi na iji ọgwụ nje mee ihe n'ụzọ sara mbara, ọrịa nosocomial nke Staphylococcus aureus na-eguzogide Methicillin (MRSA) kpatara na Staphylococcus na-arị elu.Ọnụ ọgụgụ nchọpụta mba nke MRSA bụ 30.2% na 2019 na China.E kewara MRSA n'ime nlekọta ahụike metụtara MRSA (HA-MRSA), MRSA nke obodo (CA-MRSA), na anụ ụlọ MRSA (LA-MRSA).CA-MRSA, HA-MRSA, LA-MRSA nwere nnukwu ọdịiche dị na microbiology, nje na-eguzogide ọgwụ (dịka ọmụmaatụ, HA-MRSA na-egosi ọtụtụ ọgwụ mgbochi karịa CA-MRSA) na njirimara ụlọ ọgwụ (dịka saịtị ọrịa).Dịka njirimara ndị a si dị, CA-MRSA na HA-MRSA nwere ike ịwapụta.Otú ọ dị, ọdịiche dị n'etiti CA-MRSA na HA-MRSA na-ebelata n'ihi mmegharị nke ndị mmadụ n'etiti ụlọ ọgwụ na obodo.MRSA nwere ọtụtụ ọgwụ na-eguzogide ọgwụ, ọ bụghị naanị na-eguzogide ọgwụ nje β-lactam, kamakwa aminoglycosides, macrolides, tetracyclines na quinolones ruo ogo dị iche iche.Enwere nnukwu ọdịiche mpaghara na ọnụego mgbochi ọgwụ na usoro dị iche iche.

Metcillin eguzogide mecA gene na-arụ ọrụ dị mkpa na nguzogide staphylococcal.A na-ebu mkpụrụ ndụ ihe nketa na mkpụrụ ndụ ihe nketa mobile pụrụ iche (SCCmec), nke na-etinye protein penicillin na-ekekọta 2a (PBP2a) yana ọ nwere njikọ dị ala na ọgwụ nje β-lactam, nke mere na ọgwụ nje enweghị ike igbochi njikọ nke oyi akwa peptidoglycan cell. na-akpata nkwụsị ọgwụ.

Ọwa

FAM gene mecA na-eguzogide methicillin
CY5 staphylococcus aureus nuc gene
VIC/HEX Njikwa ime

Nka nka

Nchekwa Mmiri: ≤-18℃
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị sputum, akpụkpọ ahụ na anụ ahụ dị nro, ihe nlele ọbara dum
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/ml
Nkọwapụta Ọ dịghị cross-reactivity na ndị ọzọ na-eku ume na-efe efe dị ka methicillin-sensitive staphylococcus aureus, coagulase-adịghị mma staphylococcus, methicillin na-eguzogide staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniaterbabiliater baacabiliater, clobsiella pneumoniater baacabiliater baacabiliater, klebsiella pneumoniater baacabiliater baacabiliate acinumoniae. streptococcus pneumonia , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Ngwa ndị ọdabara Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Sistemụ PCR Real-Time QuantStudio®5

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

9140713d19f7954e56513f7ff42b444


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