SARS-CoV-2 dị iche iche

Nkọwa dị mkpirikpi:

Ezubere ngwa a ka ọ bụrụ nchọpụta qualitative in vitro nke novel coronavirus (SARS-CoV-2) n'ụdị imi nasopharyngeal na oropharyngeal swab.A na-achọpụta RNA sitere na SARS-CoV-2 n'ụdị iku ume n'oge nnukwu ọrịa ma ọ bụ ndị asymptomatic.Enwere ike iji ya n'ihu nchọpụta qualitative na ọdịiche nke Alfa, Beta, Gamma, Delta na Omicron.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT072A-SARS-CoV-2 Ngwa nchọpụta iche iche (Fluorescence PCR)

Asambodo

CE

Epidemiology

Akwụkwọ akụkọ coronavirus (SARS-CoV-2) agbasawo n'ọtụtụ buru ibu gburugburu ụwa.N'ime usoro mgbasa ozi, mmụgharị ọhụrụ na-eme mgbe niile, na-ebute ụdị dị iche iche.A na-eji ngwaahịa a eme ihe maka nchọpụta inyeaka na ọdịiche nke ikpe metụtara ọrịa mgbe mgbasawanye nke Alpha, Beta, Gamma, Delta na Omicron mutant strains kemgbe Disemba 2020.

Ọwa

FAM N501Y, HV69-70del
CY5 211-212del, K417N
VIC(HEX) E484K, njikwa ime
ROX P681H, L452R

Nka nka

Nchekwa

≤-18℃ N'ọchịchịrị

Ndụ nchekwa

Ọnwa 9

Ụdị ụdị

nasopharyngeal swabs, oropharyngeal swabs

CV

≤5.0%

Ct

≤38

LoD

1000 oyiri/ml

Nkọwapụta

Enweghị mmeghari mmegharị na coronaviruse mmadụ SARS-CoV na nje ndị ọzọ na-ahụkarị.

Ngwa ngwa:

Sistemụ PCR ezigbo oge QuantStudio 5

Sistemụ PCR SLAN ®-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).

Nhọrọ 2.

Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.


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