SARS-CoV-2 dị iche iche
Aha ngwaahịa
HWTS-RT072A-SARS-CoV-2 Ngwa nchọpụta iche iche (Fluorescence PCR)
Asambodo
CE
Epidemiology
Akwụkwọ akụkọ coronavirus (SARS-CoV-2) agbasawo n'ọtụtụ buru ibu gburugburu ụwa.N'ime usoro mgbasa ozi, mmụgharị ọhụrụ na-eme mgbe niile, na-ebute ụdị dị iche iche.A na-eji ngwaahịa a eme ihe maka nchọpụta inyeaka na ọdịiche nke ikpe metụtara ọrịa mgbe mgbasawanye nke Alpha, Beta, Gamma, Delta na Omicron mutant strains kemgbe Disemba 2020.
Ọwa
| FAM | N501Y, HV69-70del |
| CY5 | 211-212del, K417N |
| VIC(HEX) | E484K, njikwa ime |
| ROX | P681H, L452R |
Nka nka
| Nchekwa | ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | nasopharyngeal swabs, oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1000 oyiri/ml |
| Nkọwapụta | Enweghị mmeghari mmegharị na coronaviruse mmadụ SARS-CoV na nje ndị ọzọ na-ahụkarị. |
| Ngwa ngwa: | Sistemụ PCR ezigbo oge QuantStudio 5 Sistemụ PCR SLAN ®-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.
