Ngwa RT-PCR fluorescent ezigbo oge maka ịchọpụta SARS-CoV-2

Nkọwa dị mkpirikpi:

Ezubere ngwa a ka in vitro qualitatively chọpụta ORF1ab na N genes of novel coronavirus (SARS-CoV-2) na nasopharyngeal swab na oropharyngeal swab anakọtara site na ikpe na ụyọkọ ikpe ndị a na-enyo enyo na ọ nwere oyi oyi bu ọrịa coronavirus ọhụrụ na ndị ọzọ achọrọ maka nyocha ahụ. ma ọ bụ nchọpụta dị iche iche nke ọrịa coronavirus ọhụrụ.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT057A-Ezigbo fluorescent RT-PCR ngwa maka ịchọpụta SARS-CoV-2

HWTS-RT057F-Freeze-akpọnwụ ezigbo ngwa RT-PCR fluorescent maka ịchọpụta SARS-CoV-2 - ngwugwu

Asambodo

CE

Epidemiology

Akwụkwọ akụkọ coronavirus (SARS-CoV-2) agbasawo n'ọtụtụ buru ibu gburugburu ụwa.N'ime usoro mgbasa ozi, mmụgharị ọhụrụ na-eme mgbe niile, na-ebute ụdị dị iche iche.A na-eji ngwaahịa a eme ihe maka nchọpụta inyeaka na ọdịiche nke ikpe metụtara ọrịa mgbe mgbasawanye nke Alpha, Beta, Gamma, Delta na Omicron mutant strains kemgbe Disemba 2020.

Ọwa

FAM 2019-nCoV ORF1ab mkpụrụ ndụ ihe nketa
CY5 Ọkpụkpụ 2019-nCoV N
VIC(HEX) mkpụrụ ndụ ihe nketa n'ime

Nka nka

Nchekwa

Mmiri: ≤-18℃ N'ọchịchịrị

Lyophilized: ≤30℃ N'ọchịchịrị

Ndụ nchekwa

Mmiri: ọnwa 9

Lyophilized: ọnwa 12

Ụdị ụdị

nasopharyngeal swabs, oropharyngeal swabs

CV

≤5.0%

Ct

≤38

LoD

300 oyiri/ml

Nkọwapụta

Enweghị mmeghari mmegharị na coronaviruse mmadụ SARS-CoV na nje ndị ọzọ na-ahụkarị.

Ngwa ngwa:

Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR SLAN ®-96P ezigbo oge

QuantStudio™ 5 Sistemụ PCR ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Nucleic Acid Mwepụta ma ọ bụ Ngwa Ọcha (Ụzọ Magnetic Beads) (HWTS-3001, HWTS-3004-32, HWTS-3004-48) sitere na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Nhọrọ 2.

Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904), Viral RNA Extraction Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd rụpụtara.


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