Mycobacterium ụkwara nta Rifampicin na-eguzogide

Nkọwa dị mkpirikpi:

Ngwa a dabara adaba maka nchọpụta qualitative nke homozygous mutation na mpaghara 507-533 amino acid codon nke mkpụrụ ndụ rpoB nke na-akpata Mycobacterium ụkwara nta rifampicin.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT074A-Mycobacterium ụkwara nta nke Rifampicin Nchọpụta Nguzogide (Fluorescence PCR)

Epidemiology

A na-eji Rifampicin na-agwọ ndị ọrịa ụkwara nta nke akpa ume kemgbe afọ 1970, ma nwee mmetụta dị ukwuu.Ọ bụ nhọrọ mbụ iji belata ọgwụgwọ ọgwụgwọ nke ndị ọrịa ụkwara nta.Ihe na-akpata nguzogide Rifampicin bụ ngbanwe nke mkpụrụ ndụ ihe nketa rpoB.Ọ bụ ezie na ọgwụ ọhụrụ na-egbochi ụkwara nta na-apụta mgbe nile, na ịdị irè ụlọ ọgwụ nke ndị ọrịa ụkwara nta na-aga n'ihu na-agakwa n'ihu, a ka nwere ụkọ ọgwụ mgbochi ụkwara nta, na ọdịdị nke iji ọgwụ na-enweghị isi eme ihe na ụlọ ọgwụ dị oke elu.N'ụzọ doro anya, ọrịa ụkwara nta nke Mycobacterium nke ndị ọrịa nwere ụkwara nta akpa ume enweghị ike igbu kpamkpam n'oge kwesịrị ekwesị, nke na-emecha na-eduga n'ọkwa dị iche iche nke nguzogide ọgwụ n'ime ahụ onye ọrịa, na-agbatị usoro nke ọrịa ahụ, na-abawanye ohere nke ọnwụ nke onye ọrịa.Ngwa a dabara adaba maka nyocha inyeaka nke ọrịa ụkwara nta nke Mycobacterium na ịchọpụta mkpụrụ ndụ ihe mgbochi rifampicin, nke na-enyere aka ịghọta nguzogide ọgwụ nke ụkwara nta mycobacterium nke ndị ọrịa butere, na inye ụzọ inyeaka maka nduzi ọgwụ ụlọ ọgwụ.

Nka nka

Nchekwa

≤-18℃ N'ọchịchịrị

Ndụ nchekwa

Ọnwa 9

Ụdị ụdị

Sputum

CV

≤5.0%

LoD

Ụdị anụ ọhịa rifampicin na-eguzogide: 2x103nje bacteria/ml

mutant homozygous: 2x103nje bacteria/ml

Nkọwapụta

Ngwa a enweghị ihe jikọrọ ya na genome mmadụ, mycobacteria ndị ọzọ na-abụghị ụkwara nta, na nje na-akpata oyi baa.Ọ na-achọpụta ebe ngbanwe nke mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ ndị ọzọ nke ụdị mycobacterium ụkwara nta dị ka katG 315G>C\A, InhA-15C> T, nsonaazụ ule na-egosi na ọ dịghị ihe mgbochi na rifampicin, nke pụtara na ọ dịghị ihe ọ bụla na-emeghachi omume.

Ngwa ngwa:

Sistemụ PCR SLAN-96P ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).

Nhọrọ 2.

Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.


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