Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa ahụ maka nchọpụta qualitative nke MERS coronavirus nucleic acid na nasopharyngeal swabs nwere Middle East Respiratory Syndrome (MERS) coronavirus.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)

Epidemiology

Ọrịa iku ume iku ume Middle East coronavirus (MERS-CoV), β-coronavirus na-akpataọrịa iku ume na ụmụ mmadụ, nke mbụ chọpụtara na onye nwụrụ anwụ 60 dị afọ Saudi Arabian nwoke ọrịa na 24th July, 2012. The clinical ngosi nke MERS-CoV ọrịa ranges si asymptomatic ala ma ọ bụ nwayọọ iku ume mgbaàmà na oké nnukwu akụkụ okuku ume ọrịa ọbụna ọnwụ.

Ọwa

FAM MERS nje virus RNA
VIC(HEX)

Njikwa ime

Nka nka

Nchekwa

≤-18℃ N'ọchịchịrị

Ndụ nchekwa

Ọnwa 9

Ụdị ụdị

Nasopharyngeal swabs anakọtara ọhụrụ

CV

≤5.0%

Ct

≤38

LoD

1000 oyiri/ml

Nkọwapụta

Enweghị mmeghachi omume ọ bụla na coronaviruses mmadụ coronavirus SARSr-CoV na nje ndị ọzọ na-ahụkarị.

Ngwa ngwa:

Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR Real-Time QuantStudio®5

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Nhọrọ 1.

Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd.

Nhọrọ 2.

Na-atụ aro mmịpụta reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


  • Nke gara aga:
  • Osote:

  • Dee ozi gị ebe a ziga anyị ya