Mmadụ ROS1 Fusion Gene Mutation

Nkọwa dị mkpirikpi:

A na-eji ngwa a mee nchọpụta qualitative in vitro nke ụdị 14 nke ROS1 fusion gene mutations na mkpụrụ ndụ cancer akpa ume mmadụ na-abụghị obere cell (Table 1).Nsonaazụ ule a bụ maka ntụaka ụlọọgwụ naanị na ekwesighi iji ya dịka naanị ihe ndabere maka ọgwụgwọ n'otu n'otu nke ndị ọrịa.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Asambodo

CE

Epidemiology

ROS1 bụ transmembrane tyrosine kinase nke ezinụlọ nnabata insulin.A kwadoro mkpụrụ ndụ ihe nketa ROS1 dị ka ihe ọzọ dị mkpa na-abụghị obere cell nke ọkwọ ụgbọ ala.Dị ka onye nnọchiteanya nke ọhụrụ pụrụ iche molekụla subtype, omume nke ROS1 fusion gene na NSCLC Ihe dị ka 1% ka 2% ROS1 na-emezigharị mkpụrụ ndụ ihe nketa na exons 32, 34, 35 na 36. Mgbe ejikọtara ya na mkpụrụ ndụ ihe nketa dịka CD74. EZR, SLC34A2, na SDC4, ọ ga-anọgide na-arụ ọrụ ROS1 tyrosine kinase mpaghara.ROS1 kinase na-arụ ọrụ na-adịghị mma nwere ike ime ka ụzọ mgbaàmà mgbada dị ka RAS/MAPK/ERK, PI3K/Akt/mTOR, na JAK3/STAT3, si otú a na-ekere òkè na mmụba, ọdịiche na metastasis nke mkpụrụ ndụ tumo, na-akpata ọrịa cancer.N'ime mmụgharị ROS1 fusion, CD74-ROS1 nwere ihe dị ka 42%, EZR bụ ihe dịka 15%, SLC34A2 bụ ihe dịka 12%, yana SDC4 nwere ihe dịka 7%.Nnyocha egosiwo na saịtị ATP-binding nke ngalaba catalytic nke ROS1 kinase na saịtị ATP-binding nke ALK kinase nwere homology ruru 77%, ya mere ALK tyrosine kinase obere molecule inhibitor crizotinib na ndị ọzọ nwere mmetụta ọgwụgwọ doro anya. na ọgwụgwọ nke NSCLC na fusion mutation nke ROS1.Ya mere, nchọpụta ROS1 fusion mutations bụ ihe ndabere na ndabere maka nduzi iji ọgwụ crizotinib eme ihe.

Ọwa

FAM Nchekwa mmeghachi omume 1, 2, 3 na 4
VIC(HEX) Ihe nchekwa mmeghachi omume 4

Nka nka

Nchekwa

≤-18℃

Ndụ nchekwa

Ọnwa 9

Ụdị ụdị

anụ ahụ na-akpata ọrịa paraffin ma ọ bụ ihe atụ nke iberibe

CV

5.0%

Ct

≤38

LoD

Ngwa a nwere ike ịchọpụta mmụgharị nke njikọ dị obere ka nnomi 20.

Ngwa ngwa:

Ngwa Biosystem 7500 ezigbo oge PCR SistemuNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR SLAN ®-96P ezigbo oge

QuantStudio™ 5 Sistemụ PCR ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Reagent mmịpụta akwadoro: Ngwa RNeasy FFPE (73504) sitere na QIAGEN, ngalaba Paraffin agbakwunyere anụ ahụ mkpokọta RNA mmịpụta ihe (DP439) sitere na Tiangen Biotech(Beijing) Co., Ltd.


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