Mmadụ CYP2C9 na VKORC1 Gene Polymorphism

Nkọwa dị mkpirikpi:

Ngwa a na-emetụta in vitro qualitative nchọpụta nke polymorphism nke CYP2C9*3 (rs1057910, 1075A>C) na VKORC1 (rs9923231, -1639G>A) na genomic DNA nke mmadụ dum ọbara samples.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-GE014A-Human CYP2C9 na VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)

Asambodo

CE

Epidemiology

Warfarin bụ ọgwụ mgbochi ọnụ nke a na-ejikarị eme ihe na ụlọ ọgwụ ugbu a, bụ nke e bu n'obi maka mgbochi na ọgwụgwọ ọrịa thromboembolic.Agbanyeghị, warfarin nwere windo ọgwụgwọ nwere oke ma dị iche iche n'etiti agbụrụ na ndị mmadụ n'otu n'otu.Statistics egosila na ọdịiche nke dose ahụ kwụsiri ike na ndị mmadụ dị iche iche nwere ike karịa ugboro 20.Ọbara ọgbụgba na-adịghị mma na-apụta na 15.2% nke ndị ọrịa na-ewere warfarin kwa afọ, nke 3.5% na-ebute ọbara ọgbụgba na-egbu egbu.Ọmụmụ ọgwụ ọgwụ egosila na mkpụrụ ndụ ihe nketa polymorphism nke lekwasịrị anya enzyme VKORC1 na enzyme metabolic CYP2C9 nke warfarin bụ ihe dị mkpa na-emetụta ọdịiche dị na dose nke warfarin.Warfarin bụ ihe mgbochi kpọmkwem nke vitamin K epoxide reductase (VKORC1), wee si otú a na-egbochi njikọ ihe na-akpata clotting nke gụnyere vitamin K ma na-enye ọgwụ mgbochi.Ọnụ ọgụgụ dị ukwuu nke ọmụmụ egosila na gene polymorphism nke VKORC1 onye na-akwalite bụ ihe kachasị mkpa na-emetụta agbụrụ na ọdịiche nke onye ọ bụla na ihe achọrọ nke warfarin.Warfarin bụ metabolized site na CYP2C9, na mutants ya na-eji nwayọọ nwayọọ na-ebelata metabolism nke warfarin.Ndị mmadụ n'otu n'otu na-eji warfarin nwere nnukwu ihe ize ndụ dị elu (ugboro abụọ ruo okpukpu atọ) nke ọbara ọgbụgba na mmalite nke ojiji.

Ọwa

FAM VKORC1 (-1639G>A)
CY5 CYP2C9*3
VIC/HEX IC

Nka nka

Nchekwa Mmiri: ≤-18℃
Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị Ọbara anticoagulated EDTA ọhụrụ
CV ≤5.0%
LoD 1.0ng/μL
Nkọwapụta Ọ dịghị cross-reactivity na ndị ọzọ na-agbanwe agbanwe usoro nke genome mmadụ (mmadụ CYP2C19 gene, mmadụ RPN2 gene);Mmụba nke CYP2C9*13 na VKORC1 (3730G>A) na-abụghị ụdị nchọpụta nke ngwa a.
Ngwa ndị ọdabara Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR Real-Time QuantStudio®5

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test akpaka Nucleic Acid Extractor (HWTS- 3006).


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