Ụdị 15 nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA

Nkọwa dị mkpirikpi:

Ngwa a bụ maka ịchọpụta qualitative 15 ọrịa papillomavirus mmadụ (HPV) E6/E7 gene mRNA okwu ọkwa n'ime mkpụrụ ndụ exfoliated nke cervix nwanyị.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-CC005A-15 Ụdị nke Papillomavirus mmadụ nwere nnukwu ihe ize ndụ E6/E7 Gene mRNA nchọpụta ngwa (Fluorescence PCR)

Epidemiology

Ọrịa kansa cervical bụ otu n'ime ụdị ọrịa kansa ụmụ nwanyị na-ahụkarị n'ụwa niile, na ihe na-eme ya nwere njikọ chiri anya na nje papillomavirus mmadụ (HPV), mana ọ bụ naanị obere akụkụ nke ọrịa HPV nwere ike ịmalite ịghọ kansa.HPV nwere nnukwu ihe ize ndụ na-ebute mkpụrụ ndụ epithelial cervical ma mepụta oncoproteins abụọ, E6 na E7.Protein a nwere ike imetụta ụdị protein cellular dị iche iche (dị ka protein suppressor pRB na p53), mee ka usoro cell dịkwuo ogologo, na-emetụta njikọ DNA na nkwụsi ike genome, ma na-egbochi nzaghachi antiviral na antitumor.

Ọwa

Ọwa Akụkụ A nwalere Genotype
FAM Ihe nchekwa mmeghachi omume HPV 1 HPV16, 31, 33, 35, 51, 52, 58
VIC/HEX Mkpụrụ ndụ β-actin mmadụ
FAM Ihe nchekwa mmeghachi omume HPV 2 HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX Mkpụrụ ndụ mmadụ INS

Nka nka

Nchekwa Mmiri: ≤-18℃
Ndụ nchekwa Ọnwa 9
Ụdị ụdị cell exfoliated cervical
Ct ≤38
CV ≤5.0%
LoD 500 oyiri/ml
Ngwa ndị ọdabara Ngwa Biosystem 7500 ezigbo oge PCR

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR Real-Time QuantStudio®5

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge

Usoro ọrụ

Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-eduzi nke ọma dị ka ntuziaka maka ojiji. .Oke elution akwadoro bụ 50μL.Ọ bụrụ na ihe nlele ahụ adịghị agbaze kpamkpam, weghachite ya na nzọụkwụ 4 maka rerigesting.Ma nwalee dịka ntuziaka maka ojiji.

Reagent mmịpụta akwadoro: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ekwesịrị ịme mmịpụta ahụ dị ka ntuziaka maka ojiji siri ike (Na nzọụkwụ 5, okpukpu abụọ nke itinye uche nke DNaseI na-arụ ọrụ ngwọta, ya bụ, were 20μL nke RNase-Free DNaseI (1500U) ngwọta ngwaahịa n'ime tube centrifuge ọhụrụ RNase-free, tinye 60μL nke ihe nchekwa RDD, ma gwakọta nwayọọ).Oke elution akwadoro bụ 60μL.Ọ bụrụ na ihe nlele ahụ agbazeghị kpamkpam, weghachite ya na nzọụkwụ 5 maka ịmegharị.Ma nwalee dịka ntuziaka maka ojiji.


  • Nke gara aga:
  • Osote:

  • Dee ozi gị ebe a ziga anyị ya