SARS-CoV-2 influenza A influenza B Nucleic Acid jikọtara
Aha ngwaahịa
HWTS-RT060A-SARS-CoV-2 influenza A influenza B Nucleic Acid Ngwa Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
Ọrịa Corona Virus 2019 (COVID-19) bụ SARS-CoV-2 nke sitere na β Coronavirus nke genus.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, igwe mmadụ na-enwekarịkwa ngwa ngwa.Ka ọ dị ugbu a, ndị ọrịa SARS-CoV-2 bu ọrịa bụ isi ihe butere ọrịa, ndị ọrịa asymptomatic nwekwara ike bụrụ isi iyi nke ọrịa.Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7.Ihe ngosi ndị bụ isi bụ ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ.Ndị ọrịa ole na ole na-enwe mgbaàmà dịka nchichi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa.
Influenza bụ nnukwu ọrịa iku ume nke nje influenza kpatara.Ọ na-efe efe nke ukwuu ma na-agbasa site na ụkwara na ọzịza.Ọ na-apụtakarị na mmiri na oyi.Enwere ụdị influenza atọ, Influenza A (IFV A), Influenza B (IFV B) na Influenza C (IFV C), ha abụọ sitere na ezinụlọ ortomyxovirus.Influenza A na B, nke nwere otu eriri, nje RNA nke akụkụ, bụ isi ihe na-ebute ọrịa mmadụ.Influenza A bụ nnukwu ọrịa na-efe efe nke iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, dị mfe ịgbanwe.ntiwapụ nke zuru ụwa ọnụ, "ngbanwe" na-ezo aka na mmụgharị nke influenza A, na-ebute nje virus ọhụrụ "subtype".A na-ekewa influenza B ụzọ abụọ: Yamagata na Victoria.Influenza B nwere naanị antigenic ịkpafu, na ha na-ezere onyunyo na mkpochapụ site na usoro ahụ ji alụso ọrịa ọgụ mmadụ site na mutation.Ma nje influenza B na-eji nwayọọ nwayọọ na-apụta karịa influenza A nke mmadụ, nke na-ebutekwa ọrịa iku ume na ọrịa na-efe efe na ụmụ mmadụ.
Ọwa
| FAM | SARS-CoV-2 |
| ROX | IFV B |
| CY5 | IFV A |
| VIC(HEX) | Mkpụrụ ndụ ihe nketa njikwa |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrị |
| Lyophilization: ≤30 ℃ n'ọchịchịrị | |
| Ndụ nchekwa | Mmiri: ọnwa 9 |
| Lyophilization: ọnwa 12 | |
| Ụdị ụdị | Nasopharyngeal swabs, Oropharyngeal swabs |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 oyiri/ml |
| Nkọwapụta | Nsonaazụ ule obe gosiri na ngwa ahụ dakọtara na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, nje syncytial respiratory A na B, nje parainfluenza 1, 2 na 3, rhinovirusA, B na C, adenovirus 1, 2, 3, 4, 5, 7 na 55, mmadụ metapneumovirus, enterovirus A, B, C na D, mmadụ cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, nje mumps, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsimigabacteriubacteria, Klebsiella tubes albicans, Candida glabrata Enweghị mmeghachi omume ọ bụla N'etiti Pneumocystis yersini na Cryptococcus neoformans. |
| Ngwa ngwa: | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.
