Otu B Streptococcus Nucleic Acid
Aha ngwaahịa
HWTS-UR010A-Nucleic Acid Nchọpụta Ngwa dabere na Enzymatic Probe Isothermal Amplification (EPIA) maka Group B Streptococcus
Epidemiology
Group B Streptococcus (GBS), nke a makwaara dị ka streptococcus agalcatiae, bụ nje na-akpata gram-positive nke na-ebikarị na traktị nri digestive dị ala na urogenital nke ahụ mmadụ.Ihe dị ka 10%-30% nke ụmụ nwanyị dị ime nwere ebe obibi GBS.Ụmụ nwanyị dị ime nwere ike ịnweta GBS n'ihi mgbanwe na gburugburu ebe obibi nke usoro ọmụmụ nke mgbanwe na ọkwa hormone dị n'ime ahụ, nke nwere ike ịkpata nsonaazụ ime ime dị njọ dị ka nnyefe akaghi aka, mgbawa nke membranes, na ịmụ nwa, ma nwekwara ike ime ya. na-ebute ọrịa puerperal na ụmụ nwanyị dị ime.Na mgbakwunye, 40%-70% nke ndị inyom bu ọrịa GBS ga-ebufe GBS nye ụmụ amụrụ ọhụrụ ha n'oge a na-amụ nwa site na oghere ọmụmụ, na-ebute ọrịa na-efe efe nke nwa ọhụrụ dị ka sepsis ọhụrụ na meningitis.Ọ bụrụ na ụmụ amụrụ ọhụrụ na-ebu GBS, ihe dịka 1%-3% n'ime ha ga-ebute ọrịa na-efe efe n'oge, na 5% ga-eduga ọnwụ.Otu nwa amụrụ ọhụrụ B streptococcus jikọtara ya na ọrịa na-efe efe na-efe efe ma bụrụ ihe dị mkpa na-ebute ọrịa na-efe efe dị ka sepsis ọhụrụ na meningitis.Ngwa a na-achọpụta n'ụzọ ziri ezi ọrịa nke otu B streptococcus iji belata ọnụọgụ na mmerụ ahụ ya na ụmụ nwanyị dị ime na ụmụ amụrụ ọhụrụ yana ibu arọ akụ na ụba na-enweghị isi nke mmerụ ahụ kpatara.
Ọwa
| FAM | GBS nucleic acid |
| ROX | ntinye aka n'ime |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | traktị genital na rectal secretions |
| Tt | .30 |
| CV | ≤10.0% |
| LoD | 500 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ genital tract na rectal swab samples dị ka Candida albicans, Trichomonas vaginalis, Chlamydia trachomatis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus, Garcoplasma genitalium, Herpes simplex virus, Garacpillomavirus, Human Paccudella virus aureus, Ntụ aka na-adịghị mma nke mba N1-N10 (Streptococcus pneumoniae, Pyogenic streptococcus, Streptococcus thermophilus, Streptococcus mutans, Streptococcus pyogenes, Lactobacillus acidophilus, Lactobacillus reuteri, Escherichia coli DH5amy DNA) |
| Ngwa ndị ọdabara | Sistemụ nchọpụta Isothermal dị mfe Amp Real-time Fluorescence (HWTS1600) Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
