Mycobacterium ụkwara nta Rifampicin na-eguzogide
Aha ngwaahịa
HWTS-RT074A-Mycobacterium ụkwara nta nke Rifampicin Nchọpụta Nguzogide (Fluorescence PCR)
Epidemiology
A na-eji Rifampicin na-agwọ ndị ọrịa ụkwara nta nke akpa ume kemgbe afọ 1970, ma nwee mmetụta dị ukwuu.Ọ bụ nhọrọ mbụ iji belata ọgwụgwọ ọgwụgwọ nke ndị ọrịa ụkwara nta.Ihe na-akpata nguzogide Rifampicin bụ ngbanwe nke mkpụrụ ndụ ihe nketa rpoB.Ọ bụ ezie na ọgwụ ọhụrụ na-egbochi ụkwara nta na-apụta mgbe nile, na ịdị irè ụlọ ọgwụ nke ndị ọrịa ụkwara nta na-aga n'ihu na-agakwa n'ihu, a ka nwere ụkọ ọgwụ mgbochi ụkwara nta, na ọdịdị nke iji ọgwụ na-enweghị isi eme ihe na ụlọ ọgwụ dị oke elu.N'ụzọ doro anya, ọrịa ụkwara nta nke Mycobacterium nke ndị ọrịa nwere ụkwara nta akpa ume enweghị ike igbu kpamkpam n'oge kwesịrị ekwesị, nke na-emecha na-eduga n'ọkwa dị iche iche nke nguzogide ọgwụ n'ime ahụ onye ọrịa, na-agbatị usoro nke ọrịa ahụ, na-abawanye ohere nke ọnwụ nke onye ọrịa.Ngwa a dabara adaba maka nyocha inyeaka nke ọrịa ụkwara nta nke Mycobacterium na ịchọpụta mkpụrụ ndụ ihe mgbochi rifampicin, nke na-enyere aka ịghọta nguzogide ọgwụ nke ụkwara nta mycobacterium nke ndị ọrịa butere, na inye ụzọ inyeaka maka nduzi ọgwụ ụlọ ọgwụ.
Nka nka
Nchekwa | ≤-18℃ N'ọchịchịrị |
Ndụ nchekwa | Ọnwa 9 |
Ụdị ụdị | Sputum |
CV | ≤5.0% |
LoD | Ụdị anụ ọhịa rifampicin na-eguzogide: 2x103nje bacteria/ml mutant homozygous: 2x103nje bacteria/ml |
Nkọwapụta | Ngwa a enweghị ihe jikọrọ ya na genome mmadụ, mycobacteria ndị ọzọ na-abụghị ụkwara nta, na nje na-akpata oyi baa.Ọ na-achọpụta ebe ngbanwe nke mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ ndị ọzọ nke ụdị mycobacterium ụkwara nta dị ka katG 315G>C\A, InhA-15C> T, nsonaazụ ule na-egosi na ọ dịghị ihe mgbochi na rifampicin, nke pụtara na ọ dịghị ihe ọ bụla na-emeghachi omume. |
Ngwa ngwa: | Sistemụ PCR SLAN-96P ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.