Mycobacterium ụkwara nta INH na-eguzogide

Ihe onyonyo nke Mycobacterium Tuberculosis INH na-eguzogide

Nkọwa dị mkpirikpi:

A na-eji ngwa a iji qualitatively chọpụta mkpụrụ ndụ ihe nketa nke 315th amino acid nke katG gene (K315G>C) na mkpụrụ ndụ ihe nketa nke mpaghara nkwalite nke mkpụrụ ndụ InhA (- 15 C>T).

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HWTS-RT002A-Mycobacterium ụkwara nta nke Isoniazid Nchọpụta Nguzogide (Fluorescence PCR)

Epidemiology

Isoniazid, bụ isi ọgwụ mgbochi ụkwara nta ewepụtara na 1952, bụ otu n'ime ọgwụ kachasị dị irè maka ọgwụgwọ ụkwara nta na-arụ ọrụ na otu ọgwụ maka ụkwara nta.

KatG bụ isi mkpụrụ ndụ ihe nketa na-edobe catalase-peroxidase na katG gene mutation nwere ike ịkwalite njikọ nke mgbidi cell mycolic acid, na-eme ka nje bacteria na-eguzogide ọgwụ isoniazid.A na-ejikọta okwu KatG n'ụzọ na-adịghị mma na mgbanwe na INH-MIC, yana mbelata okpukpu abụọ na okwu katG na-ebute mmụba dị ntakịrị okpukpu abụọ na MIC.Ihe ọzọ na-akpata nguzogide isoniazid na ụkwara nta mycobacterium na-eme mgbe ntinye ntọala, nhichapụ ma ọ bụ ngbanwe na-eme na mpaghara InhA gene nke mycobacterium ụkwara nta.

Ọwa

ROX	inhA (-15C>T) saịtị ·
CY5	katG (315G>C) saịtị
VIC (HEX)	IS6110

Nka nka

Nchekwa	≤-18℃ N'ọchịchịrị
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Sputum
CV	≤5.0%
LoD	1 × 10³nje bacteria/ml
Nkọwapụta	Mmeghachi omume enweghị obe na ngbanwe nke saịtị anọ na-eguzogide ọgwụ (511, 516, 526 na 531) nke mkpụrụ ndụ rpoB na-abụghị oke nchọta nke ngwa nchọpụta ahụ. Ngwa ngwa: Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge