MTHFR Gene Polymorphic Nucleic Acid
Aha ngwaahịa
Ngwa HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
Epidemiology
Folic acid bụ vitamin na-agbaze mmiri nke bụ ihe dị mkpa na-eme ka usoro metabolic dị n'ahụ.N'ime afọ ndị na-adịbeghị anya, ọnụ ọgụgụ dị ukwuu nke ọmụmụ ekwenyela na, mmụgharị nke folate metabolizing enzyme gene MTHFR ga-eduga na ụkọ folic acid n'ime ahụ, na mmebi nke ụkọ folic acid na ndị okenye nwere ike ime ka megaloblastic anaemia, vascular vascular. mmebi endothelial, wdg. ụkọ folic acid na ụmụ nwanyị dị ime enweghị ike igbo mkpa nke onwe ha na nwa ebu n'afọ, nke nwere ike ịkpata ntụpọ akwara akwara, anencephaly, ịmụ nwa, na ime ọpụpụ.Ọkwa ọbara folate na-emetụta 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Mmụba nke 677C>T na 1298A>C na mkpụrụ ndụ MTHFR na-ebute mgbanwe nke alanine na valine na glutamic acid, n'otu n'otu, na-ebute mbelata ọrụ MTHFR wee belata ojiji folic acid.
Ọwa
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Ọbara anticoagulated EDTA anakọtara ọhụrụ |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Ngwa ngwa: | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR SLAN ®-96P ezigbo oge QuantStudio™ 5 Sistemụ PCR ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1
Ndị na-ahụ maka mmịpụta akwadoro: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .
Nhọrọ 2
Ihe nrụpụta mmịpụta akwadoro: Ngwa mmịpụta DNA Genomic (YDP348, JCXB20210062) nke Tiangen Biotech(Beijing) Co., Ltd.Ngwa mmịpụta mkpụrụ ndụ ọbara (A1120) nke Promega.
