Papillomavirus mmadụ (ụdị 28) Genotyping
Aha ngwaahịa
HWTS-CC013-Papillomavirus mmadụ (ụdị 28) Ngwa nchọpụta Genotyping (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ahụ jọgburu onwe ya na-esikarị na akụkụ ọmụmụ ụmụ nwanyị.Nnyocha e mere n'oge gara aga egosila na ọrịa na-adịgide adịgide na ọtụtụ ọrịa papillomavirus mmadụ bụ otu n'ime ihe ndị dị mkpa na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, a ka na-enwekwaghị usoro ọgwụgwọ dị mma maka HPV, ya mere nchọpụta mmalite na mgbochi mbụ nke HPV cervical bụ igodo iji gbochie ọrịa kansa.Iji guzobe usoro nchoputa etiological dị mfe, nke akọwapụtara na ngwa ngwa dị oke mkpa na nyocha ụlọ ọgwụ nke ọrịa kansa cervical.
Ọwa
| Ihe nchekwa mmeghachi omume | FAM | VIC/HEX | ROX | CY5 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 1 | 16 | 18 | / | Njikwa ime |
| Ihe nchekwa mmeghachi omume HPV Genotyping 2 | 56 | / | 31 | Njikwa ime |
| Ihe nchekwa mmeghachi omume HPV Genotyping 3 | 58 | 33 | 66 | 35 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 4 | 53 | 51 | 52 | 45 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 5 | 73 | 59 | 39 | 68 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 6 | 6 | 11 | 83 | 54 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 7 | 26 | 44 | 61 | 81 |
| Ihe nchekwa mmeghachi omume HPV Genotyping 8 | 40 | 43 | 42 | 82 |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | cell exfoliated cervical |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300 oyiri/ml |
| Ngwa ndị ọdabara | Sistemụ PCR SLAN®-96P ezigbo oge Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Sistemụ PCR Real-Time QuantStudio®5 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler |
Usoro ọrụ
Nhọrọ 1.
Reagents mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8)
Nhọrọ 2.
Ndị na-ahụ maka mmịpụta akwadoro: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B)
