Papillomavirus mmadụ (ụdị 28) Ngwa nchọpụta Genotyping (Fluorescence PCR)

Ngwa ahụ na-eji ọtụtụ nucleic acid amplification (PCR) ụzọ nchọpụta fluorescence.A haziri primers na nyocha nke ukwuu dabere na usoro ebumnuche ebumnuche L1 nke HPV.A na-akpọ nyocha a kapịrị ọnụ na FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 4). , 45, 54, 56, 68, 82) na ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) na 5', na 3' quencher otu bụ BHQ1 ma ọ bụ BHQ2.N'oge mmụba PCR, ndị mbụ na nyocha ndị akọwapụtara na-ejikọta na usoro ebumnuche ha.Mgbe Taq enzyme zutere nyocha ndị a na-ejikọta na usoro ebumnuche, ọ na-arụ ọrụ nke 5 'njedebe exonuclease kewapụ onye nta akụkọ fluorophore na quencher fluorophore, nke mere na fluorescence nlekota usoro nwere ike ịnweta mgbaàmà fluorescent, ya bụ, oge ọ bụla DNA. A na-amụba eriri eriri, a na-emepụta molekul fluorescent, nke na-achọpụta mmekọrịta zuru oke nke nchịkọta nke akara fluorescent na nhazi nke ngwaahịa PCR, iji nweta qualitative na genotyping nchọpụta nke nucleic acids nke 28 ụdị papillomavirus mmadụ na cervical exfoliated cell samples. .