Cytomegalovirus mmadụ (HCMV) Nucleic acid
Aha ngwaahịa
HWTS-UR008A-Cytomegalovirus mmadụ (HCMV) ngwa nchọpụta nucleic acid (Fluorescence PCR)
Epidemiology
cytomegalovirus mmadụ (HCMV) bụ onye nwere genome kachasị na ezinụlọ nje virus ma nwee ike itinye ihe karịrị protein 200.HCMV ka amachibidoro ya n'ebe ndị mmadụ nọ, ma a ka nwebeghị ụdị anụmanụ nke ọrịa ya.HCMV nwere usoro mmegharị ngwa ngwa na ogologo ogologo iji mepụta ahụ ntinye intranuclear, ma kpalite mmepụta nke perinuclear na cytoplasmic nsonye ahụ na ọzịza cell (nnukwu mkpụrụ ndụ), ya mere aha ya.Dị ka heterogeneity nke genome na phenotype, HCMV nwere ike kewaa n'ime ụdị dị iche iche, n'etiti nke e nwere ụfọdụ antigenic dị iche iche, nke, Otú ọ dị, ọ dịghị mkpa ụlọ ọgwụ.
Ọrịa HCMV bụ ọrịa na-efe efe nke usoro ahụ, nke na-agụnye ọtụtụ akụkụ ahụ, nwere mgbagwoju anya na mgbaàmà dịgasị iche iche, na-emekarị ka ọ dị jụụ, ma nwee ike ime ka ndị ọrịa ole na ole nwee ọnya anụ ahụ gụnyere retinitis, ịba ọcha n'anya, oyi baa, encephalitis, colitis, monocytosis, na thrombocytopenic. purpura.Ọrịa HCMV bụ ihe a na-ahụkarị ma yie ka ọ na-agbasa n'ụwa niile.Ọ na-ewu ewu nke ukwuu na ndị bi na ya, yana ọnụ ọgụgụ omume nke 45-50% na karịa 90% na mba ndị mepere emepe na ndị na-emepe emepe, n'otu n'otu.HCMV nwere ike na-ehi ụra n'ime ahụ ruo ogologo oge.Ozugbo mgbochi ahụ na-ebelata, nje a ga-arụ ọrụ ka ọ bute ọrịa, ọkachasị ọrịa na-efe efe na-ebutekarị ndị ọrịa leukemia na ndị na-ebugharị ya, ma nwee ike ibute necrosis akụkụ ahụ a gbanyere mkpọrọgwụ ma tinye ndụ ndị ọrịa n'ọnọdụ siri ike.Na mgbakwunye na ịmụ nwa, ime ọpụpụ na nnyefe akaghi aka site na ọrịa intrauterine, cytomegalovirus nwekwara ike ịkpata mmerụ ahụ, ya mere ọrịa HCMV nwere ike imetụta nlekọta ime nwa na postnatal na àgwà ndị mmadụ.
Ọwa
FAM | DNA nke HCMV |
VIC(HEX) | Njikwa ime |
Nka nka
Nchekwa | Mmiri: ≤-18℃ N'ọchịchịrị |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | Sample Serum, Plasma Sample |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 mbipụta/mmeghachi omume |
Nkọwapụta | Ọ dịghị cross-reactivity na ịba ọcha n'anya nje, ịba ọcha n'anya C, virus papilloma mmadụ, herpes simplex virus ụdị 1, herpes simplex virus ụdị 2, nkịtị mmadụ serum samples, wdg. |
Ngwa ngwa: | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test akpaaka Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech(Beijing) Co., Ltd.