Herpes Simplex Nje Virus Ụdị 2 Nucleic Acid
Aha ngwaahịa
HWTS-UR007A-Herpes Simplex Nje Virus Ụdị 2 Nchọpụta Nucleic Acid (Fluorescence PCR)
Ezubere iji
A na-eji ngwa a maka nchọpụta qualitative nke herpes simplex virus ụdị 2 nucleic acid na nwoke urethral swab na nwanyị cervical swab sample.
Epidemiology
Herpes Simplex Virus Type 2 (HSV2) bụ nje okirikiri arụpụtara ya na tegument, capsid, isi, na envelopu, ma nwee DNA linear nwere eriri abụọ.Nje virus Herpes nwere ike ịbanye n'ime ahụ site na kọntaktị kpọmkwem ma ọ bụ mmekọahụ na akpụkpọ anụ na akpụkpọ anụ mucous, ma kewara ya na nke mbụ na nke na-emekarị.Ọrịa traktị ọmụmụ na-ebutekarị site na HSV2, ndị nwoke na-arịa ọrịa na-egosipụtakwa dị ka ọnya penile, na ndị inyom nwanyị na-egosipụta dị ka ọnya cervical, vulvar, na ikpu.Ọrịa mbụ nke nje herpes genital na-abụkarị ọrịa na-efe efe, ma e wezụga maka herpes mpaghara ole na ole nwere akpụkpọ anụ mucous ma ọ bụ akpụkpọ anụ, nke ọtụtụ n'ime ha enweghị ihe mgbaàmà ụlọ ọgwụ doro anya.Ọrịa herpes genital nwere njiri mara nje na-ebu ogologo ndụ yana ọ dị mfe nlọghachite, ma ndị ọrịa ma ndị na-ebu ya bụ isi iyi nke ọrịa ahụ.Na China, ọnụ ọgụgụ dị mma serological nke HSV2 bụ ihe dịka 10.80% ruo 23.56%.Enwere ike kewaa ọkwa nke ọrịa HSV2 gaa na ọrịa mbụ na ọrịa na-alọghachi azụ, na ihe dị ka pasent 60 nke ndị ọrịa HSV2 butere nlọghachi azụ.
Epidemiology
FAM: Herpes Simplex Nje Virus Ụdị 2 (HSV2) ·
VIC(HEX): Njikwa ime
Ntọala nkwalite PCR
Nzọụkwụ | okirikiri | Okpomọkụ | Oge | AnakọtaFluorescentSignalsma ọ bụ ọ bụghị |
1 | 1 okirikiri | 50 ℃ | 5mins | No |
2 | 1 okirikiri | 95 ℃ | Nkeji 10 | No |
3 | Usoro 40 | 95 ℃ | nkeji iri na ise | No |
4 | 58 ℃ | 31 sk | Ee |
Nka nka
Nchekwa | |
Mmiri mmiri | ≤-18℃ N'ọchịchịrị |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | Ọkpụkpụ cervical nwanyị, swab urethra nwoke |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Copy/mmeghachi omume |
Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ STD pathogens, dị ka Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium na ndị ọzọ. |
Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge. |