Herpes Simplex Nje Virus Ụdị 2 Nucleic Acid

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke herpes simplex virus ụdị 2 nucleic acid na nwoke urethral swab na nwanyị cervical swab sample.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-UR007A-Herpes Simplex Nje Virus Ụdị 2 Nchọpụta Nucleic Acid (Fluorescence PCR)

Ezubere iji

A na-eji ngwa a maka nchọpụta qualitative nke herpes simplex virus ụdị 2 nucleic acid na nwoke urethral swab na nwanyị cervical swab sample.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) bụ nje okirikiri arụpụtara ya na tegument, capsid, isi, na envelopu, ma nwee DNA linear nwere eriri abụọ.Nje virus Herpes nwere ike ịbanye n'ime ahụ site na kọntaktị kpọmkwem ma ọ bụ mmekọahụ na akpụkpọ anụ na akpụkpọ anụ mucous, ma kewara ya na nke mbụ na nke na-emekarị.Ọrịa traktị ọmụmụ na-ebutekarị site na HSV2, ndị nwoke na-arịa ọrịa na-egosipụtakwa dị ka ọnya penile, na ndị inyom nwanyị na-egosipụta dị ka ọnya cervical, vulvar, na ikpu.Ọrịa mbụ nke nje herpes genital na-abụkarị ọrịa na-efe efe, ma e wezụga maka herpes mpaghara ole na ole nwere akpụkpọ anụ mucous ma ọ bụ akpụkpọ anụ, nke ọtụtụ n'ime ha enweghị ihe mgbaàmà ụlọ ọgwụ doro anya.Ọrịa herpes genital nwere njiri mara nje na-ebu ogologo ndụ yana ọ dị mfe nlọghachite, ma ndị ọrịa ma ndị na-ebu ya bụ isi iyi nke ọrịa ahụ.Na China, ọnụ ọgụgụ dị mma serological nke HSV2 bụ ihe dịka 10.80% ruo 23.56%.Enwere ike kewaa ọkwa nke ọrịa HSV2 gaa na ọrịa mbụ na ọrịa na-alọghachi azụ, na ihe dị ka pasent 60 nke ndị ọrịa HSV2 butere nlọghachi azụ.

Epidemiology

FAM: Herpes Simplex Nje Virus Ụdị 2 (HSV2) ·

VIC(HEX): Njikwa ime

 

Ntọala nkwalite PCR

Nzọụkwụ

okirikiri

Okpomọkụ

Oge

AnakọtaFluorescentSignalsma ọ bụ ọ bụghị

1

1 okirikiri

50 ℃

5mins

No

2

1 okirikiri

95 ℃

Nkeji 10

No

3

Usoro 40

95 ℃

nkeji iri na ise

No

4

58 ℃

31 sk

Ee

Nka nka

Nchekwa  
Mmiri mmiri

≤-18℃ N'ọchịchịrị

Ndụ nchekwa Ọnwa iri na abụọ
Ụdị ụdị

Ọkpụkpụ cervical nwanyị, swab urethra nwoke

Ct

≤38

CV

≤5.0%

LoD 50Copy/mmeghachi omume
Nkọwapụta

Ọ dịghị cross-reactivity na ndị ọzọ STD pathogens, dị ka Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium na ndị ọzọ.

Ngwa ndị ọdabara

Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa.

Ngwa Biosystem 7500 ezigbo oge PCR Sistemu

Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo

Sistemụ PCR Real-Time QuantStudio®5

Sistemụ PCR SLAN-96P ezigbo oge

Sistemụ PCR Real-Time LightCycler®480

Sistemụ nchọpụta PCR nke LineGene 9600 Plus

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 ezigbo oge PCR sistemụ

BioRad CFX Opus 96 PCR ezigbo oge.

Usoro ọrụ

d7dc2562f0f3442b31c191702b7ebdc


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