Ụdị 28 nke HPV Nucleic Acid
Aha ngwaahịa
Ụdị HWTS-CC003A-28 nke HPV Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ọjọọ na-adịkarị na traktị ọmụmụ ụmụ nwanyị.Ọmụmụ ihe ahụ egosila na ọrịa na-aga n'ihu na ọtụtụ ọrịa papillomavirus mmadụ bụ otu n'ime ihe ndị dị mkpa na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, a ka enwebeghị usoro ọgwụgwọ dị mma maka HPV.Ya mere, nchọpụta mmalite na mgbochi mbụ nke HPV cervical bụ isi ihe na-egbochi ọrịa cancer.Ịmepụta usoro nchọpụta ọrịa dị mfe, kpọmkwem na ngwa ngwa dị oke mkpa na nchọpụta ụlọ ọgwụ nke ọrịa cancer cervical.
Ọwa
S/N | Ọwa | Ụdị |
PCR-Mix1 | FAM | 16, 18, 31, 56 |
VIC(HEX) | Njikwa ime | |
CY5 | 45, 51, 52, 53 | |
ROX | 33, 35, 58, 66 | |
PCR-Mix2 | FAM | 6, 11, 54, 83 |
VIC(HEX) | 26, 44, 61, 81 | |
CY5 | 40, 42, 43, 82 | |
ROX | 39, 59, 68, 73 |
Nka nka
Nchekwa | ≤-18℃ n'ọchịchịrị |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | Selụ exfoliated cervical |
Ct | ≤28 |
CV | ≤5.0 nkeji |
LoD | 300 oyiri/ml |
Nkọwapụta | Ọ dịghị cross-reactivity na nkịtị ọmụmụ tract pathogens (dị ka ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, ebu, gardnerella na ndị ọzọ HPV ụdị adịghị ekpuchi na kit, wdg). |
Ngwa ndị ọdabara | Ọ nwere ike dabara na ngwa PCR fluorescent nke dị n'ahịa. Sistemụ PCR ABI 7500 ezigbo oge QuantStudio® 5 Sistemụ PCR ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler |
Ngwọta PCR zuru oke
Nhọrọ 1.
![Fluorescence PCR3](http://www.hongweitest.com/uploads/Fluorescence-PCR3.jpg)
Nhọrọ 2.
![Fluorescence PCR4](http://www.hongweitest.com/uploads/Fluorescence-PCR4.jpg)