Ụdị 28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18 typing) Nucleic acid
Aha ngwaahịa
Ụdị HWTS-CC006A-28 nke Nje Virus papilloma mmadụ nwere nnukwu ihe ize ndụ (16/18) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ahụ jọgburu onwe ya na-enwekarị n'ime akụkụ ọmụmụ ụmụ nwanyị.Nnyocha egosiwo na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, ọgwụgwọ ndị a ma ama dị irè ka enweghị maka ọrịa kansa cervical nke HPV kpatara, yabụ nchọpụta mmalite na mgbochi ọrịa cervical nke HPV kpatara bụ isi ihe na-egbochi ọrịa kansa cervical.Ọ bụ ihe dị oke mkpa iji guzobe nnwale diagnostic dị mfe, nke akọwapụtara na ngwa ngwa maka nyocha ụlọ ọgwụ na ọgwụgwọ ọrịa kansa cervical.
Ọwa
| Ngwakọta mmeghachi omume | Ọwa | Ụdị |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Njikwa ime | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | Mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | cell exfoliated cervical |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Sistemụ PCR Real-Time QuantStudio®5 Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 PCR ozugbo Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Ndị na-emegharị mmịpụta akwadoro: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Nhọrọ 2.
Reagents mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Ngwa Ọcha(YDP315) nke Tiangen Biotech (Beijing) Co., Ltd.
